By cultivation-independent specific gene probes screening two bacteria groups were analysed as dominant bacteria in paper mill samples. Detected bacteria groups were related to Tepidimonas arfidensis (member of the Betaproteobacteria) and Cloacibacterium (member of the Cytophaga-Flexibacter subphylum), respectively. In samples from SAPPI both groups together represented between 56% and 86% of all viable bacteria. Retrieved results not only confirm our findings in an earlier EU-project (“ODOUR CONTROL”- No.: 016730) but confirm findings of recent studies considering these two bacteria groups together with Rhodobacter species as the early biofilm forming bacteria in samples from paper mills with neutral to alkaline process conditions.

According to these preliminary results it is likely that Tepidimonas arfidensis and Cloacibacterium may have a high potential to act as indicator organisms for biofilm formation in paper mill samples with neutral to alkaline process conditions. The developed specific probes will be used as monitoring tools for screening further paper mill samples. If these results are confirmed the developed probes for these microorganisms will be transferred into easy-to-use test kits for general use as early biofilm monitoring tools in paper mills. Such new kits will have a wide potential market.

Process-flow to understanding the microbiology in the four industries (green rhombi indicate the key goals of the microbiological analysis

Overview on the steps of the VIT-Analysis

Monitoring bacteria in textile industry
The analyses of the samples from Svilanit and Texktina revealed an astonishing view into the microbiology of process streams in textile factories. The combination of VIT-profiling together with the bio-digital-approach allowed a deep look into the black box of unknown and non-cultivable bacteria and presented an exciting microbial diversity. To our present knowledge there exist until now only minor investigations regarding microbiology in textile factories but never has anyone succeeded in retrieving the "whole picture". Within this project we will be able to present the first step of a complete approach for understanding microbiology in textile factories. That means not only in distinct steps of the different processes but from input water to process water to wastewater. Only with this approach we will be able to understand the full picture of the textile-correlated microbiology. The AquaFit4Use project will without doubts present the worldwide deepest look into microbiology of textile companies and their process streams.

Do you know that...........

Cultivation-based methods can only show up a maximum of 0.1% - 15% of all micro-organisms in a certain environment.

184 micro-organisms from literature and experimental data have been selected so far within Aquafit4use as being considered relevant for water streams within the four sectors? Based on the gained knowledge in this project a prioritization on further probe development is been performed by vermicon.

62 gene probes are available for cultivation-independent monitoring of important micro-organisms directly in water samples of the four industries. These probes detect pathogens and other microorganisms supposed to be relevant for processes as well as in hygiene, biofilm and biofouling aspects.

Claudia Beimfohr (VER)

Based on report on Microbiology in AQUAFIT4USE
 

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